Hitachi McDATA Sphereon 4500 User Manual download pdf

HITACHI 4500-Il INSTRUCTIONS

The following instructions provide a guide to performing simple tasks on the microscope. Initial sections

(A to D) describe how to insert a sample and obtain an image. Subsequent sections provide information

on more COni~!CX operations such as changing the accelerating voltage or the detecton Sections K and

L provide infonnation on what to do at the end of a session.

Compared with the JEOL 840 SEM there are more variable parameters available to the operator.

Optimum imaging conditions will vary from user to user depending on their samples. It is

advisable that you spend some time attempting to determine which conditions are best for your

sample.

When using this microscope please note the following rules:

RULE 1: DON'T TOUCH A CONTROL IF YOU ARE NOT SURE OF THE

OUTCOME OF THAT ACTION.

RULE 2: NEVER, EVER FORCE ANYTHING BEYOND FINGER STRENGTH

RULE 3: IF IN DOUBT; ASK FOR HELP

A. START-UP

1. Turn on the visual display by turning toggle switch DISPLAY POWER to 1. (These

toggle switches must be pulled out slightly before switching - DO NOT FORCE THEM)

2. Turn the toggle switch SC AIRLOCK VALVE from CLOSED to AUTO (OPEN)- a

few seconds later the green light on the HV off/ready display will come on. If it doesn't please

contact EM staff for help.

B. INSERT SAMPLE

1. Put specimen stub onto specimen holder> inc exact mechanism will vary depending upon

the specimen type. EM Unit staff will advise the best method for your sample when you art

being trained. The specimen holder and stub MUST be set so that so that thtt top of the

specimen is level with or below tIze gauge.

2. Ensure toggle switch on SC AIRLOCK VALVE DISPLAY is set to S.E.C.

3. Press AIR on Sc AIRLOCK VALVE DISPLAY - this will bleed air into the specimen

exchange chamber. It will take 2~3O seconds for the chamber to come to air.

4. Ensure stage settings (X,Y,Z,R and T) have been return to their default positions

(displayed on the specimen chamber).

5. Open specimen exchange chamber door Do not force.

6. Push specimen exchange rod forward slightly so it protrudes through the specimen exchange

chamber door and screw specimen holder on to screw thread of the specimen exchange rod.

PLEASE MAKE SURE YOU DO NOT GET A CROSS THREAD. Pull specimen

exchange rod back into specimen exchange chamber door.

7. Close airlock door

1 2 3 4 5 6

Summary of Contents

Page 1 - HITACHI 4500-Il INSTRUCTIONS

HITACHI 4500-Il INSTRUCTIONS The following instructions provide a guide to performing simple tasks on the microscope. Initial sections (A to D) descri

Page 2

8. Press EVAC on SC AIRLOCK VALVE DISPLAY - this will pump the specimen exchange chamber. 9. Observe SEC vacuum display as status lights show vacuum

Page 3

D. TAKING A PHOTOGRAPH 1. Obtain the image you desire. 2. Press ABC button on the image adjust section to automatically alter brightness and cont

Page 4 - G. CHANGING THE DETECTOR

10. Hit ENTER to move $ sign against STIG ALIGN Y Press ENTER again 11. The image will oscillate on the screen, minimise oscillation with ALIGNMENT X

Page 5 - FORCE THIS LEVER

I. LOW MAGNIFICATION MODE Often it can be difficult to locate the area you want on a specimen. In this case use Low Magnification Imaging, here the

Page 6 - L. FINISHING A SESSION

L. FINISHING A SESSION 1. Turn the SC AIRLOCK VALVE from AUTO (OPEN) to CLOSED - the green light on the HV off/ready display will turn off.. 2. T

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Hitachi McDATA Sphereon 4500 User Manual

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